Test Code RMCCSPUTUM Sputum Culture*
Methodology
Culture/Gram Stain
Performing Laboratory
Rutland Regional Medical Center Laboratory
Specimen Requirements
Submit only 1 of the following specimens:
Specimen Type: Expectorated sputum
Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Collect under direct supervision of nurse or physician.
2. Have patient remove dentures, if appropriate.
3. Instruct patient to take a deep breath, hold it momentarily, then cough deeply and vigorously into container, and collect expectorated material.
Specimen Type: Induced sputum
Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Have patient remove dentures, if appropriate.
2. Instruct patient to brush his/her teeth and/or rinse mouth well with water.
3. With aid of nebulizer, have patient inhale approximately 25 mL of 3% to 10% sterile saline.
4. Collect expectorated material in container.
Specimen Transport Temperature
Ambient/Refrigerated NO/Frozen NO
Day(s) Performed
Daily
Analytical Time:
3 days
Test Classification and CPT Coding
CPT: 87070
87205-Gram stain
LOINC: 6460-0
Order Code: RMCCSPUTUM
Result Code: RMCL800057
Result Name: Sputum Culture