Clostridioides difficile PCR*
Performing Laboratory
Rutland Regional Medical Center Laboratory
Specimen Requirements
Specimen Type: Only liquid/unformed stools are acceptable
Container/Tube:
Place liquid or soft stool specimen in a sterile cup/container with a secure lid.
Specimen Volume: 0.5 mL
Specimen Minimum Volume: 0.5 mL
Collection Instructions:
Pass liquid or soft (assumes shape of container) stool directly into a clean, dry container.
Additional Information:
1. Patient should be passing ≥5 stools per 24 hours with liquid or soft consistency.
2. Submit only 1 specimen per 7 days.
3. If a patient is positive for C. difficile no additional specimens will be accepted for 10 days.
4. This assay is not approved to test for cure since nucleic acids may persist after effective treatment.
5. Up to 50% of neonates may be colonized with toxigenic C. difficile. Testing of children less than 1 year of age is not recommended.
Day(s) Performed
Daily
Analytical Time:
1 day
Test Classification and CPT Coding
CPT: 87493
LOINC: 34713-8
Order Code: RMCCDIFTOX
Result Code: RMCL802746
Result Name: Clostridioides difficile PCR
Methodology
DiaSorin Molecular Simplexa™ C. difficile Direct assay using real time PCR.
Specimen Stability
Refrigerated up to 48 hours
Reference Values
Negative
Significant value (Results called on all inpatients/institutional residents. All outpatient results called during business hours only.)