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Venous Blood Gas

Important Note

Deliver specimen on wet ice within 30 minutes.

Performing Laboratory

Rutland Regional Medical Center Laboratory

Specimen Requirements

Specimen must arrive within 30 minutes of draw.

 

Specimen Type: Whole blood-Clotted blood is not acceptable.
Container/Tube: Heparinized blood gas syringe

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL
Collection Instructions:

1. Draw arterial blood anaerobically.

2. Expel air bubbles, remove needle from syringe and cap-Air bubble is not acceptable.

3. Immediately place syringe on wet ice.

 

Specimen Type: Whole blood-Clotted blood is not acceptable.
Container/Tube: Dark-green (lithium heparin) Vacutainer

Specimen Volume: 2 mL

Collection Instructions:

1. Draw arterial blood anaerobically.

2. Expel air bubbles and cap-Air bubble is not acceptable.

3. Immediately place tube on wet ice.

Days Performed

Daily

Analytical Time:

1 hour

Available Stat

Methodology

Ion-Selective Electrode (ISE)
Includes pH, pCO2, pO2, O2 saturation (SO2), base excess, and bicarbonate (HCO3).

Test Classification and CPT Coding

82803

Normal Reference Values

pH

7.38-7.46

 

pCO2

32-46 mm Hg

 

pO2

74-108 mm Hg

 

SO2 %

92%-96% saturation

 

BASE EXCESS

-3.0 - +3.0 mmol/L

 

HCO3

24-32 mEq/L

Specimen Transport Temperature

On wet ice

Critical Values

pH

<7.2 or >7.6 (automatic call-back)

 

pCO2

<20 mm Hg or >70 mm Hg (automatic call-back)

 

pO2

<50 mm Hg (automatic call-back)

 

HCO3

<15 mEq/L (automatic call-back)